ELISA HiPure DNA|RNA|Protein

1 HiPure DNA/RNA/Protein KitR5211 Cat. # PRODUCT NAME Quantity PRICE R521102 HiPure DNA/RNA/Protein Kit 50 preps $273.00 R521103 HiPure DNA/RNA/Protein Kit 250 preps $1,193.00 Introduction Hipure DNA / RNA / protein kit is the most rapid and simple method to simµLtaneously extract DNA, RNA and protein from cµLtured cells and animal soft tissue samples. This kit adopts column technology which is no need for dangerous phenol chloroform extraction and time-consuming alcohol precipitation in the extraction process. This kit is suitable for simµLtaneous extraction of RNA, DNA and protein from < 1x 10^7 eukaryotic cµLtured cells as well as from animal soft tissues less than 20mg such asliver, kidney, spleen and brain. The purified RNA can be directly used in RT-PCR, Northern blot, poly-A + purification, nucleic acid protection, in vitro translation while the obtained DNA can be directly used in PCR and Southern hybridization, the obtained protein can be directly used in SDS-PAGE electrophoresis, western blot and so on. Details Specifications Features Specifications Main Functions Co-isolation DNA and RNA /protein from a single sample(cells,tissue, plant sample) Applications SDS-PAGE electrophoresis and western blot, etc Purification method Mini spin column Purification technology Silica technology Process method Manual (centrifµgation or vacuum) Sample type CµLture cells, animal tissues, plant fungi,yeast, bacteria and other samples Sample amount CµLtured cells: < 10^7 Animal soft tissue: ≤ 30 mg Plant/fungal samples with less secondary metabolites: ≤ 100 mg Yeast cells: 2 x 10^6 - 5 x 10^7 Yield Elution volume Time per run Liquid carrying volume per column Binding yield of column Principle Advantages High quality - high purity total RNA / DNA can be directly used in a variety of downstream applications Fast - column method can complete the extraction of several samples in 30 minutes Safe - no phenol chloroform extraction required SimµLtaneous extraction- simµLtaneously isolate DNA and RNA from one sample Kit Contents Contents R521102 R521103 Number of Purification 50 Preps 250 Preps HiPure DNA Mini Columns II 50 250 HiPure RNA Mini Columns 50 250 2mL Collection Tubes 100 500 Buffer RL 50 mL 250 mL Buffer DW1 30 mL 150 mL Buffer RW1 50 mL 250 mL Buffer RW2* 2 x 20 mL 3 x 50 mL RNase Free Water 10 mL 30 mL Elution Buffer 10 mL 30 mL DPS Solution (5% SDS) 30 mL 150 mL Storage and Stability Hipure DNA/RNA/protein kit can be stored at room temperature (15-25℃) in dry for 18 months. For long-term storage, it shall be stored at 2-8℃. At low temperature, buffer RL may form precipitation. It needs 55℃ water bath to completely dissolve the precipitation before use. As DEPC treated water does not contain any bacteriostatic factors, it may be contaminated by bacterial or fungal when placed or operated at room temperature. We recommend to pack DEPC treated water separately and store it at -20℃ to reduce pollution. If DEPC treated water is polluted, please prepare it again.