ELISA Ribonuclease Inhibitor

Ribonuclease Inhibitor, Placenta is a recombinant placental protein with specifically inhibits broad-spectrum RNase such as RNase A, RNase B and RNase C. It is not effective against RNase 1, RNase T1, S1 Nuclease, RNase H. It does not inhbit Taq DNA polymerase and T7 RNA Polymerase. The 50kDa protein exerts its inhibitory effect by noncovalently binding to RNases at 1:1 ratio. The Ki value for binding of Rnase Inhibitor, Placenta to RNase is approximately 10(-14)M. Feature: Improved resistance to oxidation, compared to /porcine Rnase inhibitor Ideal for reactions where low DTT concentrations are required (e.g. Real-time PCR) Source An E.coli strain that carries Ribonuclease Inhibitor gene from placenta. Volume activity 40U/µL Source An E.coli strain that carries Ribonuclease Inhibitor gene from placenta. Recombinant Volume activity 40U/µL Unit Definition of Rnase in One unit is defined as the amount of Recombinant Ribonuclease Inhibitor required inhibit the activity of 5ng of ribonuclease A by 50%. Activity is measured by the inhibition of hydrolysis of cytidine 2’3’-cyclic monophosphate by ribonuclease A. Storage Temperature -20°C Storage Buffer 20mM HEPES-KOH(PH7.6), 50mM KCl, 8mM DTT, 50%(v/v) glycerol. Quality Control: Absence of Endonuclease 1μg of Lambda DNA is incubated with 200 units of Ribonuclease Inhibitor for 16 hour at 37°C. Following incubation, Lambda DNA is visualized as intact on an ethidium bromide-stained agarose gel to verify the absence of visible Endonuclease. RNase Inhibitor, Murine is a 50 kDa recombinant protein of murine origin. The inhibitor specifically inhibits RNases A, B and C. It inhibits RNases by binding noncovalently in a 1:1 ratio with high affinity. It is not effective against RNase 1, RNase T1, S1 Nuclease, RNase H or RNase from Aspergillus. In addition, no inhibition of polymerase activity is observed when RNase Inhibitor is used with Taq DNA Polymerase, AMV or M-MµLV Reverse Transcriptases, or Phage RNA Polymerases (SP6, T7, or T3).