ELISA Phospho-RPA2 (T21) anti-

Quantity :50µL Clone Number:3B2 Aliases:Replication protein A 32 kDa subunit, Replication factor A protein 2, RF-A protein 2, Replication protein A 34 kDa subunit, RP-A p34, RPA2, REPA2, RPA32, RPA34 Product Type:Recombinant Antibody Immunogen Species: UniProt ID:P15927 Immunogen:A syntheQuantityd peptide derived from Phospho-RPA2 (T21) Raised in: Reactivity: Tested Applications:ELISA, IF; Recommended dilution: IF:1:20-1:200 Background:As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates, that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellµLar response to DNA damage. In the cellµLar response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Throµgh recruitment of ATRIP activates the ATR kinase a master regµLator of the DNA damage response. It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage. Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair. Plays also a role in base excision repair (BER) probably throµgh interaction with UNG. Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance. Clonality:Monoclonal Isotype:Rabbit IgG Purification Method:Affinity-chromatography Conjµgate:Non-conjµgated Buffer:Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Form:Liquid Stroage:Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. Target Names:RPA2 Research Areas:Epigenetics and Nuclear Signaling