ELISA DNA2 anti-

Quantity :50µg Clone Number: Aliases:DNA replication ATP-dependent helicase-like homolog antibody; DNA replication helicase 2 homolog antibody; DNA replication helicase 2, yeast, homolog of antibody; DNA replication helicase 2-like antibody; DNA replication helicase/nuclease 2 antibody; DNA2 antibody; DNA2-like helicase antibody; DNA2L antibody; DNA2L_ antibody; HDNA2 antibody; KIAA0083 antibody; PEOA6 antibody; SCKL8 antibody Product Type:Polyclonal Antibody Immunogen Species:Homo sapiens () UniProt ID:P51530 Immunogen:Recombinant DNA replication ATP-dependent helicase/nuclease DNA2 protein (1-300AA) Raised in:Rabbit Reactivity: Tested Applications:ELISA, IHC; Recommended dilution: IHC:1:20-1:200 Background:Key enzyme involved in DNA replication and DNA repair in nucleus and mitochondrion. Involved in Okazaki fragments processing by cleaving long flaps that escape FEN1: flaps that are longer than 27 nucleotides are coated by replication protein A complex (RPA), leading to recruit DNA2 which cleaves the flap until it is too short to bind RPA and becomes a substrate for FEN1. Also involved in 5\'-end resection of DNA during double-strand break (DSB) repair: recruited by BLM and mediates the cleavage of 5\'-ssDNA, while the 3\'-ssDNA cleavage is prevented by the presence of RPA. Also involved in DNA replication checkpoint independently of Okazaki fragments processing. Possesses different enzymatic activities, such as single-stranded DNA (ssDNA)-dependent ATPase, 5\'-3\' helicase and endonuclease activities. While the ATPase and endonuclease activities are well-defined and play a key role in Okazaki fragments processing and DSB repair, the 5\'-3\' DNA helicase activity is subject to debate. According to various reports, the helicase activity is weak and its function remains largely unclear. Helicase activity may promote the motion of DNA2 on the flap, helping the nuclease function. Clonality:Polyclonal Isotype:IgG Purification Method:>95%, Protein G purified Conjµgate:Non-conjµgated Buffer:Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 Form:Liquid Stroage:Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. Target Names:DNA2 Research Areas:Epigenetics and Nuclear Signaling