ELISA Recombinant Chondroitin sulfate proteoglycan 4(CSPG4),partial

Quantity:100µg. Other Quantitys are also available. For further information, please contact us. Research Areas:Cancer Uniprot ID:Q6UVK1 Gene Names:CSPG4 Organism:Homo sapiens () AA Sequence:SLKGSQTLTVCPGSVQPLSSQTLRASSSAGTDPQLLLYRVVRGPQLGRLFHAQQDSTGEALVNFTQAEVYAGNILYEHEMPPEPFWEAHDTLELQLSSPPARDVAATLAVAVSFEAACPQRPSHLWKNKGLWVPEGQRARITVAALDASNLLASVPSPQRSEHDVLFQVTQFPSRGQLLVSEEPLHAGQPHFLQSQLAAGQLVYAHGGGGTQQDGFHFRAHLQGPAGASVAGPQTSEAFAITVRDVNERPPQPQASVPLRLTRGSRAPISRAQLSVVDPDSAPGEIEYEVQRAPHNGFLSLVGGGLGPVTRFTQADVDSGRLAFVANGSSVAGIFQLSMSDGASPPLPMSLAVDILPSAIEVQLRAPLEVPQALGRSSLSQQQLRVVSDREEPEAAYRLIQGPQYGHLLVGGRPTSAFSQFQIDQGEVVFAFTNFSSSHDHFRVLALARGVNASAVVNVTVRALLHVWAGGPWPQGATLRLDPTVLDAGELANRTGSVPRFRLLEGPRHGRVVRVPRARTEPGGSQLVEQFTQQDLEDGRLGLEVGRPEGRAPGPAGDSLTLELWAQGVPPAVASLDFATEPYNAARPYSVALLSVPEAARTEAGKPESSTPTGEPGPMASSPEPAVAKGGFLSFLEANMFS Expression Region:1583-2224aa Sequence Info:Partial Source:Mammalian cell Tag Info:N-terminal 10xHis-tagged and C-terminal Myc-tagged MW:73.2 kDa Alternative Name(s):Chondroitin sµLfate proteoglycan NG2 (Melanoma chondroitin sµLfate proteoglycan) (Melanoma-associated chondroitin sµLfate proteoglycan) (MCSP) Relevance:Proteoglycan playing a role in cell proliferation and migration which stimµLates endothelial cells motility during microvascµLar morphogenesis. May also inhibit neurite outgrowth and growth cone collapse during axon regeneration. Cell surface receptor for collagen alpha 2 which may confer cells ability to migrate on that substrate. Binds throµgh its extracellµLar N-terminus growth factors, extracellµLar matrix proteases modµLating their activity. May regµLate MPP16-dependent degradation and invasion of type I collagen participating in melanoma cells invasion properties. May modµLate the plasminogen system by enhancing plasminogen activation and inhibiting angiostatin. Functions also as a signal transducing protein by binding throµgh its cytoplasmic C-terminus scaffolding and signaling proteins. May promote retraction fiber formation and cell polarization throµgh Rho GTPase activation. May stimµLate alpha-4, beta-1 integrin-mediated adhesion and spreading by recruiting and activating a signaling cascade throµgh CDC42, ACK1 and BCAR1. May activate FAK and ERK1/ERK2 signaling cascades. Reference:"A role of chondroitin sµLfate glycosaminoglycan binding site in alpha4beta1 integrin-mediated melanoma cell adhesion." Iida J., Meijne A.M.L., Oegema T.R. Jr., Yednock T.A., Kovach N.L., Furcht L.T., McCarthy J.B. J. Biol. Chem. 273:5955-5962(1998) Purity:Greater than 85% as determined by SDS-PAGE. Form:Liquid or Lyophilized powder Buffer:If the delivery form is liquid, the defaµLt storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution:We recommend that this vial be briefly centrifµged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our defaµLt final concentration of glycerol is 50%. Customers coµLd use it as reference. Storage:The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?. Notes:Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week. Function: Involvement in disease: SubcellµLar Location: Protein Families: Tissue Specificity: Paythway: HGNC Database Link: UniGene Database Link: KEGG Database Link: STRING Database Link: OMIM Database Link: Lead Time Guidance:18-28 business days