ELISA Mouse Dystrophia myotonica WD repeat-containing protein (DMWD)

Reactivity:Mouse (Mus muscµLus) UniProt:Q08274 Abbreviation:DMWD Alternative Names:D19S593E; DMR-N9; DMRN9; gene59; dystrophia myotonica-containing WD repeat motif|dystrophia myotonica-containing WD repeat motif protein|protein 59 Application:ELISA Range:Request Information Sensitivity:Request Information Intra-AssayCV:?6.0% Inter-AssayCV:?10.1% Recovery:1.09 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate DMWD in samples. An antibody specific for DMWD has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDMWD present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for DMWD is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DMWD bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:DMWD contains 2 regions with significant homology to WD repeats. Northern blot analysis detected highest expression in mouse brain and testis, with significant expression in heart and lung. In situ hybridization of mouse embryos detected ubiquitous low expression in all tissues and enhanced expression in adµLt brain and testis. Dmwd expression began before embryonic day 9.5. From day 14.5 onward, Dmwd mRNA was detected in all neural tissues, especially in the telencephalon and mesencephalon. Later, expression was evident in distinct tubµLes of the mature testis and was restricted to secondary spermatocytes of stages VIII to XII of the spermatogenic proliferation cycle.Northern blot analysis of and baboon RNA detected a 3-kb transcript. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).