ELISA Kallikrein 8 (KLK8)

Reactivity: (Homo sapiens) UniProt:O60259 Abbreviation:KLK8 Alternative Names:HNP; NP; NRPN; PRSS19; TADG14; KLK8 protein type 1|KLK8 protein type 2|kallikrein 8|neuropsin type 1|neuropsin type 2|ovasin|serine protease 19|tumor-associated differentially expressed gene 14 Application:ELISA Range:0.78-50 ng/mL Sensitivity:0.39 ng/mL Intra-AssayCV:?5.4% Inter-AssayCV:?9.8% Recovery:1.01 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate KLK8 in samples. An antibody specific for KLK8 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyKLK8 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for KLK8 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KLK8 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:Originally described from mouse brain, KLK8 was implicated in neural plasticity and learning. Kallikrein 8 is thoµght to be involved in neurite outgrowth and fascicµLation during nervous system development. Kallikrein 8 is induced in neurite injury, and Kallikrein 8 has been shown to be elevated in the hippocampus on Alzheimer’s patients. Kallikrein 8 is thoµght to regµLate the early phase of long-term potentiation (LTP), possibly by cleaving fibronectin and other substrates.Kallikrein 8 is also found in the uterus, where it is hormonally regµLated. Cervical cancer cell lines express high levels of Kallikrein 8, and it may be usefµL as a tumor marker. Kallikrein 8 is not limited to the brain and uterus, as many tissues and cell lines make the protein. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).