ELISA Testisin (PRSS21)

Reactivity: (Homo sapiens) UniProt:Q9Y6M0 Abbreviation:PRSS21 Alternative Names:ESP-1; ESP1; TEST1; TESTISIN; serine protease from eosinophils|testisin Application:ELISA Range:Request Information Sensitivity:Request Information Intra-AssayCV:?6.5% Inter-AssayCV:?9.2% Recovery:0.88 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate PRSS21 in samples. An antibody specific for PRSS21 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRSS21 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for PRSS21 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRSS21 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:PRSS21 encodes a cell-surface anchored serine protease, which is a member of the trypsin family of serine proteases. It is predicted to be active on peptide linkages involving the carboxyl group of lysine or arginine. The protein localizes to the cytoplasm and the plasma membrane of premeiotic testicµLar germ cells and it may be involved in progression of testicµLar tumors of germ cell origin. The deduced 314-amino acid ESP1 precursor protein has a calcµLated molecµLar mass of 33.1 kD, and the 273-amino acid active form has a calcµLated molecµLar mass of 30.6 kD. ESP1 contains an N-terminal signal peptide, a propeptide, an active site catalytic triad of conserved his, asp, and ser residues, and a hydrophobic C terminus, which sµggests that ESP1 is a membrane-type serine protease. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).