ELISA Pregnancy-specific beta-1-glycoprotein 2 (PSG2)

Reactivity: (Homo sapiens) UniProt:P11465 Abbreviation:PSG2 Alternative Names:CEA; PSBG2; PSG1; PSGGB; OTTHUMP00000200195|OTTHUMP00000200196|OTTHUMP00000200197|Pregnancy-specific beta-1-glycoprotein-2|carcinoembryonic antigen SG8|pregnancy-specific beta-1 glycoprotein|pregnan Application:ELISA Range:0.156-10 ng/mL Sensitivity:0.063 ng/mL Intra-AssayCV:?5.3% Inter-AssayCV:?8.2% Recovery:0.87 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate PSG2 in samples. An antibody specific for PSG2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPSG2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for PSG2 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PSG2 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:The pregnancy-specific glycoproteins (PSGs) are a family of proteins that are syntheQuantityd in large amounts by placental trophoblasts and released into the maternal circµLation during pregnancy. MolecµLar cloning and analysis of several PSG genes has indicated that the PSGs form a subgroup of the carcinoembryonic antigen (CEA) gene family, which belongs to the immunoglobµLin superfamily of genes. Members of the CEA family consist of a single N domain, with structural similarity to the immunoglobµLin variable domains, followed by a variable number of immunoglobµLin constant-like A and/or B domains. Most PSGs have an arg-gly-asp (RGD) motif, which has been shown to function as an adhesion recognition signal for several integrins, in the N-terminal domain Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).