ELISA Sodium- and chloride-dependent glycine transporter 1 (SLC6A9)

Reactivity: (Homo sapiens) UniProt:P48067 Abbreviation:SLC6A9 Alternative Names:DKFZp547A1118; GLYT1; sodium- and chloride-dependent glycine transporter 1|solute carrier family 6 member 9 Application:ELISA Range:0.312-20 ng/mL Sensitivity:0.134 ng/mL Intra-AssayCV:?5.3% Inter-AssayCV:?8.1% Recovery:0.92 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate SLC6A9 in samples. An antibody specific for SLC6A9 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySLC6A9 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for SLC6A9 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLC6A9 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:Glycine transport is mediated by 2 sodium-dependent carriers, GLYT1 and GLYT2, that have distinct tissue distributions. While the 3-prime sequences of these 2 cDNAs were identical, the 5-prime noncoding regions and the N termini were completely different. GLYT1b was found only in the white matter of the CNS, while GLYT1a was found in the gray matter of the CNS as well as in macrophages and mast cells in peripheral tissues. Tissue-specific alternative splicing or alternative promoter usage from a single gene resµLted in 2 mRNA products encoding similar but distinct glycine transporters. The anatomic distribution of GLYT1a mRNA supported the emerging status of glycine as a supraspinal neurotransmitter and sµggested that glycine may function as a chemical messenger outside the CNS Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).