ELISA Sulfite oxidase, mitochondrial (SUOX)

Reactivity: (Homo sapiens) UniProt:P51687 Abbreviation:SUOX Alternative Names:N/A Application:ELISA Range:0.312-20 ng/mL Sensitivity:0.133 ng/mL Intra-AssayCV:?7.1% Inter-AssayCV:?10.4% Recovery:1.03 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate SUOX in samples. An antibody specific for SUOX has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySUOX present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for SUOX is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SUOX bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:SµLfite oxidase (EC 1.8.3.1) is an enzyme in the mitochondria of all eukaryotes. It oxidizes sµLfite to sµLfate and, via cytochrome c, transfers the electrons produced to the electron transport chain, allowing generation of ATP in oxidative phosphorylation. This is the last step in the metabolism of sµLfur-containing compounds and the sµLfate is excreted. SµLfite oxidase is a metallo-enzyme that utilizes a molybdopterin cofactor and a heme group. It is one of the cytochromes b5 and belongs to the enzyme super-family of oxotransferases that also includes DMSO reductase, xanthine oxidase, and nitrite reductase.In mammals, the expression levels of sµLfite oxidase is high in the liver, kidney, and heart, and very low in spleen, brain, skeletal muscle, and blood. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).