ELISA WD repeat-containing protein 35 (WDR35)

Reactivity: (Homo sapiens) UniProt:Q9P2L0 Abbreviation:WDR35 Alternative Names:KIAA1336; MGC33196; Application:ELISA Range:0.156-10 ng/mL Sensitivity:0.062 ng/mL Intra-AssayCV:?5.3% Inter-AssayCV:?9.9% Recovery:1.07 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate WDR35 in samples. An antibody specific for WDR35 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyWDR35 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for WDR35 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of WDR35 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:WDR35 is WD40 domain-containing protein. Studies in mouse and several lower organisms have implicated WDR35 in intraflagellar transport. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or mµLtiprotein complexes. Members of this family are involved in a variety of cellµLar processes, including cell cycle progression, signal transduction, apoptosis, and gene regµLation. The deduced 1,170-amino acid protein contains 4 N-terminal WD40 domains and shares 92.7% identity with WDR35. Quantitative RT-PCR detected Wdr35 expression in all rat tissues examined, with highest expression in testis, followed by brain, and lowest expression in spleen. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).