ELISA FlyCut® HindIII

Product Details FlyCut® HindIII is expressed and purified from E.coli that carries the recombinant HindIII gene. The molecµLar weight is 35.8kDa, with the recognition site at A^AGCTT. The reaction is conducted for 5-15 minutes at 37℃, and heat-inactivated at 65℃ for 20 minutes. This enzyme is not sensitive to dam, dcm or mammalian CpG methylation. Recognition Site 5’...A↓AGCTT...3’ 3’...TTCGA↑A...5’ Applications Genomic DNA, plasmid DNA, PCR product Storage at -20°C for two years Shipping Dry ice (-70 ℃) Product Contents Component JH101-01 JH101-02 FlyCut® HindIII 5,000 units 2×5,000 units 10×FlyCut® Buffer 2×1 mL 3×1 mL 10×DNA Loading Buffer 1 mL 1 mL