ELISA FlyCut® EcoRI

Product Details FlyCut® EcoRI is expressed and purified from E.coli that carries the recombinant EcoRI gene. The molecµLar weight is 32.0 kDa, with the recognition site at G^AATTC. The reaction is conducted for 5-15 minutes at 37℃, and heat-inactivated at 65℃ for 20 minutes. This enzyme is not sensitive to dam, dcm methylation, but sensitive to mammalian CpG methylation. Recognition Site 5’...G↓AATTC...3’ 3’...CTTAA↑G...5’ Applications Genomic DNA, plasmid DNA, PCR product Storage at -20°C for two years Shipping Dry ice (-70 ℃) Product Contents Component JE201-01 JE201-02 FlyCut® EcoRI 5,000 units 2×5,000 units 10×FlyCut® Buffer 2×1 mL 3×1 mL 10×DNA Loading Buffer 1 mL 1 mL