ELISA Phospho-STAT1 (S727) anti-

Quantity :50µL Clone Number:2H10 Aliases:Signal transducer and activator of transcription 1-alpha/beta, Transcription factor ISGF-3 components p91/p84, STAT1 Product Type:Recombinant Antibody Immunogen Species: UniProt ID:P42224 Immunogen:A syntheQuantityd peptide derived from Phospho-STAT1 (S727) Raised in: Reactivity: Tested Applications:ELISA, WB, IHC, IF; Recommended dilution: WB:1:500-1:5000, IHC:1:50-1:200, IF:1:20-1:200 Background:Signal transducer and transcription activator that mediates cellµLar responses to interferons (IFNs), cytokine KITLG/SCF and other cytokines and other growth factors. Following type I IFN (IFN-alpha and IFN-beta) binding to cell surface receptors, signaling via protein kinases leads to activation of Jak kinases (TYK2 and JAK1) and to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize and associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus (PubMed:28753426). ISGF3 binds to the IFN stimµLated response element (ISRE) to activate the transcription of IFN-stimµLated genes (ISG), which drive the cell in an antiviral state. In response to type II IFN (IFN-gamma), STAT1 is tyrosine- and serine-phosphorylated (PubMed:26479788). It then forms a homodimer termed IFN-gamma-activated factor (GAF), migrates into the nucleus and binds to the IFN gamma activated sequence (GAS) to drive the expression of the target genes, inducing a cellµLar antiviral state. Becomes activated in response to KITLG/SCF and KIT signaling. May mediate cellµLar responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Clonality:Monoclonal Isotype:Rabbit IgG Purification Method:Affinity-chromatography Conjµgate:Non-conjµgated Buffer:Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Form:Liquid Stroage:Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. Target Names:STAT1 Research Areas:Signal Transduction