ELISA DCAF1 anti-

Quantity :50µg Clone Number: Aliases:DCAF1 antibody; KIAA0800 antibody; RIP antibody; VPRBPDDB1- and CµL4-associated factor 1 antibody; HIV-1 Vpr-binding protein antibody; VprBP antibody; Serine/threonine-protein kinase VPRBP antibody; EC 2.7.11.1 antibody; Vpr-interacting protein antibody Product Type:Polyclonal Antibody Immunogen Species:Homo sapiens () UniProt ID:Q9Y4B6 Immunogen:Recombinant DDB1- and CµL4-associated factor 1 protein (647-745AA) Raised in:Rabbit Reactivity: Tested Applications:ELISA, IHC, IF; Recommended dilution: IHC:1:20-1:200, IF:1:50-1:200 Background:Acts both as a substrate recognition component of E3 ubiquitin-protein ligase complexes and as an atypical serine/threonine-protein kinase, playing key roles in various processes such as cell cycle, telomerase regµLation and histone modification. Probable substrate-specific adapter of a DCX (DDB1-CµL4-X-box) E3 ubiquitin-protein ligase complex, named CµL4A-RBX1-DDB1-DCAF1/VPRBP complex, which mediates ubiquitination and proteasome-dependent degradation of proteins such as NF2. Involved in the turnover of methylated proteins: recognizes and binds methylated proteins via its chromo domain, leading to ubiquitination of target proteins by the RBX1-DDB1-DCAF1/VPRBP complex (PubMed:23063525). The CµL4A-RBX1-DDB1-DCAF1/VPRBP complex is also involved in B-cell development: VPRBP is recruited by RAG1 to ubiquitinate proteins, leading to limit error-prone repair during V(D)J recombination. Also part of the EDVP complex, an E3 ligase complex that mediates ubiquitination of proteins such as TERT, leading to TERT degradation and telomerase inhibition (PubMed:23362280). Also acts as an atypical serine/threonine-protein kinase that specifically mediates phosphorylation of \\\'Thr-120\\\' of histone H2A (H2AT120ph) in a nucleosomal context, thereby repressing transcription. H2AT120ph is present in the regµLatory region of many tumor suppresor genes, down-regµLates their transcription and is present at high level in a number of tumors (PubMed:24140421). Involved in JNK-mediated apoptosis during cell competition process via its interaction with LLGL1 and LLGL2 (PubMed:20644714). In case of infection by HIV-1 virus, it is recruited by HIV-1 Vpr in order to hijack the CµL4A-RBX1-DDB1-DCAF1/VPRBP function leading to arrest the cell cycle in G2 phase, and also to protect the viral protein from proteasomal degradation by another E3 ubiquitin ligase. The HIV-1 Vpr protein hijacks the CµL4A-RBX1-DDB1-DCAF1/VPRBP complex to promote ubiquitination and degradation of proteins such as TERT and ZIP/ZGPAT. In case of infection by HIV-2 virus, it is recruited by HIV-2 Vpx in order to hijack the CµL4A-RBX1-DDB1-DCAF1/VPRBP function leading to enhanced efficiency of macrophage infection and promotion of the replication of cognate primate lentiviruses in cells of monocyte/macrophage lineage. Clonality:Polyclonal Isotype:IgG Purification Method:>95%, Protein G purified Conjµgate:Non-conjµgated Buffer:Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 Form:Liquid Stroage:Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. Target Names:DCAF1 Research Areas:Microbiology