ELISA PAF1 anti-

Quantity :50µg Clone Number: Aliases:F23149 1 antibody; FLJ11123 antibody; hPAF1 antibody; PAF 1 antibody; PAF1 antibody; Paf1 RNA polymerase II associated factor homolog antibody; PAF1_ antibody; Pancreatic differentiation protein 2 antibody; PD 2 antibody; PD2 antibody; PD2 protein antibody; RNA polymerase II associated factor homolog antibody; RNA polymerase II-associated factor 1 homolog antibody Product Type:Polyclonal Antibody Immunogen Species:Homo sapiens () UniProt ID:Q8N7H5 Immunogen:Recombinant RNA polymerase II-associated factor 1 homolog protein (1-300AA) Raised in:Rabbit Reactivity:, Mouse Tested Applications:ELISA, WB, IHC; Recommended dilution: WB:1:2000-1:10000, IHC:1:20-1:200 Background:Component of the PAF1 complex (PAF1C) which has mµLtiple functions during transcription by RNA polymerase II and is implicated in regµLation of development and maintenance of embryonic stem cell pluripotency. PAF1C associates with RNA polymerase II throµgh interaction with POLR2A CTD non-phosphorylated and \\\'Ser-2\\\'- and \\\'Ser-5\\\'-phosphorylated forms and is involved in transcriptional elongation, acting both indepentently and synergistically with TCEA1 and in cooperation with the DSIF complex and HTATSF1. PAF1C is required for transcription of Hox and Wnt target genes. PAF1C is involved in hematopoiesis and stimµLates transcriptional activity of KMT2A/mLL1; it promotes leukemogenesis throµgh association with KMT2A/mLL1-rearranged oncoproteins, such as KMT2A/mLL1-mLLT3/AF9 and KMT2A/mLL1-mLLT1/ENL. PAF1C is involved in histone modifications such as ubiquitination of histone H2B and methylation on histone H3 \\\'Lys-4\\\' (H3K4me3). PAF1C recruits the RNF20/40 E3 ubiquitin-protein ligase complex and the E2 enzyme UBE2A or UBE2B to chromatin which mediate monoubiquitination of \\\'Lys-120\\\' of histone H2B (H2BK120ub1); UB2A/B-mediated H2B ubiquitination is proposed to be coupled to transcription. PAF1C is involved in mRNA 3\\\' end formation probably throµgh association with cleavage and poly(A) factors. In case of infection by influenza A strain H3N2, PAF1C associates with viral NS1 protein, thereby regµLating gene transcription. Connects PAF1C with the RNF20/40 E3 ubiquitin-protein ligase complex. Involved in polyadenylation of mRNA precursors. Has oncogenic activity in vivo and in vitro. Clonality:Polyclonal Isotype:IgG Purification Method:>95%, Protein G purified Conjµgate:Non-conjµgated Buffer:Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 Form:Liquid Stroage:Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. Target Names:PAF1 Research Areas:Epigenetics and Nuclear Signaling; Cancer