ELISA RNF168 Antibody, HRP

Quantity :50µg Clone Number: Aliases:E3 ubiquitin protein ligase RNF168 antibody; E3 ubiquitin-protein ligase RNF168 antibody; FLJ35794 antibody; FLJ39749 antibody; hRNF168 antibody; RING finger protein 168 antibody; RN168_ antibody; RNF 168 antibody; Rnf168 antibody Product Type:Polyclonal Antibody Immunogen Species:Homo sapiens () UniProt ID:Q8IYW5 Immunogen:Recombinant E3 ubiquitin-protein ligase RNF168 protein (194-296AA) Raised in:Rabbit Reactivity: Tested Applications:ELISA Background:E3 ubiquitin-protein ligase required for accumµLation of repair proteins to sites of DNA damage. Acts with UBE2N/UBC13 to amplify the RNF8-dependent histone ubiquitination. Recruited to sites of DNA damage at double-strand breaks (DSBs) by binding to ubiquitinated histone H2A and H2AX and amplifies the RNF8-dependent H2A ubiquitination, promoting the formation of \'Lys-63\'-linked ubiquitin conjµgates. This leads to concentrate ubiquitinated histones H2A and H2AX at DNA lesions to the threshold required for recruitment of TP53BP1 and BRCA1. Also recruited at DNA interstrand cross-links (ICLs) sites and promotes accumµLation of \'Lys-63\'-linked ubiquitination of histones H2A and H2AX, leading to recruitment of FAAP20/C1orf86 and Fanconi anemia (FA) complex, followed by interstrand cross-link repair. H2A ubiquitination also mediates the ATM-dependent transcriptional silencing at regions flanking DSBs in cis, a mechanism to avoid collision between transcription and repair intermediates. Also involved in class switch recombination in immune system, via its role in regµLation of DSBs repair. Following DNA damage, promotes the ubiquitination and degradation of JMJD2A/KDM4A in collaboration with RNF8, leading to unmask H4K20me2 mark and promote the recruitment of TP53BP1 at DNA damage sites. Not able to initiate \'Lys-63\'-linked ubiquitination in vitro; possibly due to partial occlusion of the UBE2N/UBC13-binding region. Catalyzes monoubiquitination of \'Lys-13\' and \'Lys-15\' of nucleosomal histone H2A (H2AK13Ub and H2AK15Ub, respectively). Clonality:Polyclonal Isotype:IgG Purification Method:>95%, Protein G purified Conjµgate:HRP Buffer:Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 Form:Liquid Stroage:Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. Target Names:RNF168 Research Areas:Epigenetics and Nuclear Signaling