ELISA Phospho-NFKB2 (Ser870) anti-

Quantity :100µL Clone Number: Aliases:CVID10 antibody; DNA binding factor KBF2 antibody; DNA-binding factor KBF2 antibody; H2TF1 antibody; Lymphocyte translocation chromosome 10 antibody; Lymphocyte translocation chromosome 10 protein antibody; Lyt 10 antibody; Lyt10 antibody; NF kB2 antibody; NFKB2 antibody; NFKB2_ antibody; Nuclear factor NF kappa B p100 subunit antibody; Nuclear factor NF kappa B p52 subunit antibody; Nuclear factor NF-kappa-B p52 subunit antibody; Nuclear factor of kappa light chain gene enhancer in B cells 2 antibody; Nuclear factor of kappa light polypeptide gene enhancer in B cells 2 antibody; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 2 antibody; Oncogene Lyt 10 antibody; Oncogene Lyt-10 antibody; p105 antibody; p49/p100 antibody Product Type:Polyclonal Antibody Immunogen Species:Homo sapiens () UniProt ID:Q00653 Immunogen:Peptide sequence around phosphorylation site of serine 870(Y-G-S(p)-Q-S) derived from NF?B-p100. Raised in:Rabbit Reactivity:, Mouse, Rat Tested Applications:ELISA, WB, IHC, IF; WB:1:500-1:1000, IHC:1:50-1:100, IF:1:100-1:200 Background: NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimµLi related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellµLar compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. In a non-canonical activation pathway, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-kappa-B RelB-p52 complexes. The NF-kappa-B heterodimeric RelB-p52 complex is a transcriptional activator. The NF-kappa-B p52-p52 homodimer is a transcriptional repressor. NFKB2 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p100 and generation of p52 by a cotranslational processing. The proteasome-mediated process ensures the production of both p52 and p100 and preserves their independent function. p52 binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. p52 and p100 are respectively the minor and major form; the processing of p100 being relatively poor. Isoform p49 is a subunit of the NF-kappa-B protein complex, which stimµLates the HIV enhancer in synergy with p65.Dobrzanski P., Ryseck R.P., Bravo R.EMBO J. 13:4608-4616(1994)Betts J.C., Nabel G.J.Mol. Cell. Biol. 16:6363-6371(1996) Clonality:Polyclonal Isotype:IgG Purification Method:Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjµgates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi Conjµgate:Non-conjµgated Buffer:Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Form:liquid Stroage:Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. Target Names:NFKB2 Research Areas:Epigenetics and Nuclear Signaling;Cancer;Cell biology;Signal transduction