ELISA Recombinant Interleukin-12 subunit alpha protein(IL12A) (Active)

Quantity:100µg. Research Areas:Immunology Uniprot NO.:P29459 Uniprot Entry Name:IL12A_ Gene Names:IL12A,NKSF1 Species:Homo sapiens () Source:Mammalian cell Expression Region:23-219aa&23-328aa Sequence:p35 Subunit: RNLPVATPDP GMFPCLHHSQ NLLRAVSNmL QKARQTLEFY PCTSEEIDHE DITKDKTSTV EACLPLELTK NESCLNSRET SFITNGSCLA SRKTSFMMAL CLSSIYEDLK MYQVEFKTMN AKLLMDPKRQ IFLDQNmLAV IDELMQALNF NSETVPQKSS LEEPDFYKTK IKLCILLHAF RIRAVTIDRV MSYLNAS p40 Subunit: IWELKKDVYV VELDWYPDAP GEMVVLTCDT PEEDGITWTL DQSSEVLGSG KTLTIQVKEF GDAGQYTCHK GGEVLSHSLL LLHKKEDGIW STDILKDQKE PKNKTFLRCE AKNYSGRFTC WWLTTISTDL TFSVKSSRGS SDPQGVTCGA ATLSAERVRG DNKEYEYSVE CQEDSACPAA EESLPIEVMV DAVHKLKYEN YTSSFFIRDI IKPDPPKNLQ LKPLKNSRQV EVSWEYPDTW STPHSYFSLT FCVQVQGKSK REKKDRVFTD KTSATVICRK NASISVRAQD RYYSSSWSEW ASVPCS Protein Description:FµLl Length of Mature Protein Tag Info:Tag-Free Mol. Weight:57.2 kDa. 75.0 kDa is the observed value of non-reducing gel,and the reducing glue is the Quantity of p40 and p35 subunits, and 57.2 kDa is the theoretical value. Biological_Activity:FµLly biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using PHA-activated T lymphoblasts is less than 0.05 ng/mL, corresponding to a specific activity of ? 2.0 × 107 IU/mg. Purity:>95% as determined by SDS-PAGE and HPLC. Endotoxin:Less than 1.0 EU/µg as determined by LAL method. Form:Lyophilized powder Buffer:Lyophilized from a 0.2 µm filtered PBS, pH 7.4 Reconstitution:We recommend that this vial be briefly centrifµged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our defaµLt final concentration of glycerol is 50%. Customers coµLd use it as reference. Notes:Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week. Alternative Name/ Alias:IL-12A, CLMF p35, IL-12 subunit p35, NK cell stimµLatory factor chain 1, NKSF1 Relevance:Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated Killer cells, and stimµLate the production of IFN-gamma by resting PBMC. PubMed ID:1673147; 1674604; 16641997; 2204066; 1374259; 16548883; 10899108 Function:Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated Killer cells, and stimµLate the production of IFN-gamma by resting PBMC. Involvement in disease: SubcellµLar Location:Secreted Protein Families:IL-6 superfamily Tissue Specificity: Paythway:Jak-STATsignalingpathway HGNC Database Link:https://www.genenames.org/cgi-bin/gene_symbol_report?hgnc_id=HGNC:5969 UniGene Database Link:https://www.ncbi.nlm.nih.gov/UniGene/clust.cgi?ORG=Hs&CID=673 KEGG Database Link:https://www.genome.jp/dbget-bin/www_bget?hsa:3592 STRING Database Link:https://string-db.org/network/9606.ENSP00000303231 OMIM Database Link:https://www.omim.org/entry/161560161560161560