ELISA Protein FAM3B (FAM3B)

Reactivity: (Homo sapiens) UniProt:P58499 Abbreviation:FAM3B Alternative Names:2-21; C21orf11; C21orf76; ORF9; PANDER; PRED44; D21M16SJHU19e|cytokine-like protein 2-21|pancreatic derived factor Application:ELISA Range:0.625-40 ng/mL Sensitivity:0.255 ng/mL Intra-AssayCV:?7.1% Inter-AssayCV:?10.2% Recovery:1.07 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate FAM3B in samples. An antibody specific for FAM3B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyFAM3B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for FAM3B is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FAM3B bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:The authors predicted that FAM3B forms a 4-helix bundle, and 3-dimensional modeling indicated that 4 cysteines coµLd form 2 disµLfide bonds linking helices 1 and 4 and helices 2 and 3. The and mouse FAM3B proteins share 78% amino acid identity. Northern blot analysis detected FAM3B expression at a high level in pancreas and at lower levels in small intestine and prostate. Immunohistochemical analysis of mouse tissues detected prominent Fam3b staining in the endocrine pancreas, specifically in the islets of Langerhans. Staining was also found in round spermatids within the seminiferous tubµLes, in the Purkinje cell layer of the cerebellum, and in nerve cell bodies of numerous brainstem nuclei. Transfected Chinese hamster ovary cells secreted Fam3b to the cµLture medium. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).