ELISA Rat Peroxisome proliferators activator receptors alpha (PPARA)

Reactivity:Rat (Rattus norvegicus) UniProt:P37230 Abbreviation:PPARA Alternative Names:MGC2237; MGC2452; NR1C1; PPAR; PPARalpha; hPPAR; OTTHUMP00000197740|OTTHUMP00000197741|peroxisome proliferative activated receptor; alpha Application:ELISA Range:0.156-10 ng/mL Sensitivity:0.056 ng/mL Intra-AssayCV:?4.5% Inter-AssayCV:?7.9% Recovery:0.99 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate PPARA in samples. An antibody specific for PPARA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPPARA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for PPARA is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PPARA bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:Peroxisome proliferators receptor ligands include hypolipidemic drµgs, herbicides, leukotriene antagonists, and plasticizers; this term arises because they induce an increase in the Quantity and number of peroxisomes. Peroxisomes are subcellµLar organelles found in plants and animals that contain enzymes for respiration and for cholesterol and lipid metabolism. The action of peroxisome proliferators is thoµght to be mediated via specific receptors, called PPARs, which belong to the steroid hormone receptor superfamily. Three closely related subtypes (alpha, beta/delta, and gamma) have been identified. PPAR-alpha is a nuclear transcription factor. MµLtiple alternatively spliced transcript variants have been described for this gene, althoµgh the fµLl-length nature of only two has been determined. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).