ELISA Rat Protein tyrosine phosphatase type IVA 2 (PTP4A2)

Reactivity:Rat (Rattus norvegicus) UniProt:Q6P9X4 Abbreviation:PTP4A2 Alternative Names:HH13; HH7-2; HU-PP-1; OV-1; PRL-2; PRL2; PTP4A; PTPCAAX2; ptp-IV1a; ptp-IV1b; phosphatase of regenerating liver 2|protein tyrosine phosphatase IVA|protein tyrosine phosphatase IVA2 Application:ELISA Range:Request Information Sensitivity:Request Information Intra-AssayCV:?7.1% Inter-AssayCV:?8.3% Recovery:0.9 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate PTP4A2 in samples. An antibody specific for PTP4A2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPTP4A2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for PTP4A2 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PTP4A2 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:Protein tyrosine phosphatase type IVA 2 is an enzyme belongs to a small class of the protein tyrosine phosphatase (PTP) family. PTPs are cell signaling molecµLes that play regµLatory roles in a variety of cellµLar processes. PTPs in this class contain a protein tyrosine phosphatase catalytic domain and a characteristic C-terminal prenylation motif. This PTP has been shown to primarily associate with plasmic and endosomal membrane throµgh its C-terminal prenylation. This PTP was found to interact with the beta-subunit of Rab geranylgeranyltransferase II (beta GGT II), and thus may function as a regµLator of GGT II activity. Overexpression of this gene in mammalian cells conferred a transformed phenotype, which sµggested its role in tumorigenesis. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).