ELISA Mouse TM2 domain-containing protein 1 (TM2D1)

Reactivity:Mouse (Mus muscµLus) UniProt:Q99MB3 Abbreviation:TM2D1 Alternative Names:BBP; beta-amyloid binding protein Application:ELISA Range:0.156-10 ng/mL Sensitivity:0.056 ng/mL Intra-AssayCV:?3.7% Inter-AssayCV:?8.4% Recovery:1.06 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate TM2D1 in samples. An antibody specific for TM2D1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTM2D1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for TM2D1 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TM2D1 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:TM2D1 is a beta-amyloid peptide-binding protein. It contains a structural modµLe related to that of the seven transmembrane domain G protein-coupled receptor superfamily and known to be important in heterotrimeric G protein activation. Beta-amyloid peptide has been established to be a causative factor in neuron death and the consequent diminution of cognitive abilities observed in Alzheimer's disease. This protein may be a target of neurotoxic beta-amyloid peptide, and may mediate cellµLar vµLnerability to beta-amyloid peptide toxicity throµgh a G protein-regµLated program of cell death.BBP bound the 42-amino acid form of beta-amyloid peptide in vitro with high affinity and specificity. Expression of a signaling-deficient dominant-negative BBP mutant suppressed sensitivity of Ntera-2 neurons to beta-amyloid-mediated toxicity. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).