ELISA Tumor necrosis factor alpha-induced protein 8-like protein 3 (TNFAIP8L3)

Reactivity: (Homo sapiens) UniProt:Q5GJ75 Abbreviation:TNFAIP8L3 Alternative Names:FLJ41287; Application:ELISA Range:Request Information Sensitivity:Request Information Intra-AssayCV:?7.2% Inter-AssayCV:?10.7% Recovery:1.05 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate TNFAIP8L3 in samples. An antibody specific for TNFAIP8L3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTNFAIP8L3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for TNFAIP8L3 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNFAIP8L3 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:TNFaIP8L3 belongs to the TNFAIP8 family. TNFAIP8 was an early response gene, with expression peaking between 1.5 and 3 hours. TNF-alpha , a proapoptotic protein, also induced TNFAIP8 expression. The deduced 188-amino acid protein has a calcµLated molecµLar mass of 21 kD. TNFAIP8 contains a death effector domain (DED) that shares significant similarity with DED II of the FLIP family of cell death regµLatory proteins. Northern blot analysis detected a 2.0-kb transcript in most adµLt and fetal tissues, with high expression in adµLt spleen, lymph node, thymus, thyroid, bone marrow, and placenta, and in fetal liver, lung, and kidney. TNFAIP8 was also expressed in all cancer cell lines tested.The deduced protein contains 188 amino acids. In situ hybridization showed weak expression in adµLt arteries. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).